| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pendrin; Sodium-independent chloride/iodide transporter; Solute carrier family 26 member 4; SLC26A4; PDS |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SLC26A4, which shares 84.4% amino acid (aa) sequence identity with both mouse and rat SCNN1A. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects pendrin (gene SLC26A4) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 85.7 kDa, and the supplier reports an observed band at about 86 kDa.
Pendrin (SLC26A4) is an anion exchanger that moves chloride, iodide and bicarbonate across the apical membrane of epithelial cells in the thyroid, inner ear and kidney. It helps release iodide into the thyroid follicle and controls fluid and pH in the inner ear, and its mutations cause Pendred syndrome and a common form of inherited hearing loss.
| Target | Pendrin (gene SLC26A4; UniProt O43511, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 85.7 kDa |
| Observed MW | 86 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human HK-2 cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human SLC26A4. Sequence identity with the mouse and rat orthologs: 84.4%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places pendrin in the cell membrane and apical cell membrane. Tissue expression noted by UniProt (human): Highly expressed in the kidney (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
