Anti-SLC27A4 / FATP4 Rabbit Monoclonal Antibody

SKU:BHA21010081
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-SLC27A4 antibody (Rabbit monoclonal, clone 25S88, IgG). Validated for Western blot, immunofluorescence and flow cytometry. Reactivity: Human, Mouse, Rat. Recognizes long-chain fatty acid transport protein 4.
Target SLC27A4
clone number 25S88
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB, ICC, IF, Flow
Options selector
Catalog no. Size Conjugation
M05299 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,ICC,IF,Flow Cytometry
      Application details: WB 1:500-2000<br>ICC/IF 1:50-200<br>FC 1:100<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target SLC27A4
Alternative names Long-chain fatty acid transport protein 4; FATP-4; Fatty acid transport protein 4; Arachidonate--CoA ligase; Long-chain-fatty-acid--CoA ligase; Solute carrier family 27 member 4; Very long-chain acyl-CoA synthetase 4; ACSVL4; SLC27A4; FATP4
UniProt # Q6P1M0
Host Rabbit
Clone 25S88
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunofluorescence
  • Flow Cytometry
Immunogen A synthesized peptide derived from human SLC27A4 / FATP4
Molecular weight 72.1 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cell membrane, sarcolemma; Single-pass type II membrane protein. Cytoplasm, cytoskeleton.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M05299
Main SKU BHA21010081
Rabbit Monoclonal

Product Overview

Clone 25S88 is a rabbit monoclonal antibody that recognizes long-chain fatty acid transport protein 4 (gene SLC27A4) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 72.1 kDa, and the supplier reports an observed band at about 72 kDa.

FATP4 (SLC27A4) is a fatty acid transport protein and acyl-CoA synthetase that helps cells import long-chain fatty acids by activating them to CoA esters. It is the main fatty acid transporter in the small intestine, is important for skin barrier formation, and its mutations cause ichthyosis prematurity syndrome.

Target Long-chain fatty acid transport protein 4 (gene SLC27A4; UniProt Q6P1M0, human)
Clone 25S88
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 72.1 kDa
Observed MW 72 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunocytochemistry / Immunofluorescence 1:50–1:200
Flow cytometry 1:100

Samples with a confirmed band (WB): HeLa cells, mouse kidney.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human SLC27A4 / FATP4.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places long-chain fatty acid transport protein 4 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Expressed at highest levels in brain, testis, colon and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:100. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 72.1 kDa; observed band at about 72 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human SLC27A4 / FATP4.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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