| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium-dependent phosphate transport protein 2B; Sodium-phosphate transport protein 2B; Na (+)-dependent phosphate cotransporter 2B; NaPi3b; Sodium/phosphate cotransporter 2B; Na (+)/Pi cotransporter 2B; NaPi-2b; Solute carrier family 34 member 2; SLC34A2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SLC34A2, which shares 83.3% amino acid (aa) sequence identity with both mouse and rat SLC34A2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes sodium-dependent phosphate transport protein 2B (gene SLC34A2) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 75.8 kDa, and the supplier reports an observed band at about 130 kDa.
NaPi-IIb (SLC34A2) is a sodium-dependent phosphate cotransporter. It is the main route for phosphate absorption in the small intestine and is also expressed in lung alveolar type II cells, where it clears phosphate from the alveolar fluid; its mutations cause pulmonary alveolar microlithiasis.
| Target | Sodium-dependent phosphate transport protein 2B (gene SLC34A2; UniProt O95436, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 75.8 kDa |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human 293T cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, mouse lung tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human SLC34A2. Sequence identity with the mouse and rat orthologs: 83.3%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sodium-dependent phosphate transport protein 2B in the apical cell membrane. Tissue expression noted by UniProt (human): Highly expressed in lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xuehao Zhu et al. (2025) N6-methyladenosine on L1PA governs the trans-silencing of LTRs and restrains totipotency in naive human embryonic stem cells. Cell Stem Cell. 10.1016/j.stem.2025.10.003
