| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nucleotide sugar transporter SLC35D1; Solute carrier family 35 member D1; UDP-galactose transporter-related protein 7; UGTrel7; UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter; UDP-GlcA/UDP-GalNAc transporter; SLC35D1; KIAA0260 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLC35D1 recombinant protein (Position: M1-V355). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets nucleotide sugar transporter SLC35D1 in human and mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 39.2 kDa, and the supplier reports an observed band at about 39 kDa.
SLC35D1 is a nucleotide sugar transporter in the endoplasmic reticulum membrane. It moves UDP-glucuronic acid and UDP-N-acetylgalactosamine into the ER, supplying sugars for chondroitin sulfate synthesis, and its loss causes Schneckenbecken dysplasia, a lethal skeletal disorder.
| Target | Nucleotide sugar transporter SLC35D1 (gene SLC35D1; UniProt Q9NTN3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 39.2 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SIHA cells, human Daudi cells, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human SLC35D1 fragment (Met1–Val355), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places nucleotide sugar transporter SLC35D1 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
