| Field | Specification |
|---|---|
| Target | |
| Alternative names | Zinc transporter ZIP10; Solute carrier family 39 member 10; Zrt- and Irt-like protein 10; ZIP-10; SLC39A10; KIAA1265; ZIP10 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLC39A10 recombinant protein (Position: H29-H379). Human SLC39A10 shares 77.9% amino acid (aa) sequence identity with mouse SLC39A10. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes zinc transporter ZIP10 (gene SLC39A10) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 94.1 kDa, and the supplier reports an observed band at about 95–100 kDa.
ZIP10 (SLC39A10) is a zinc importer that moves zinc from outside the cell into the cytosol. It forms a heterodimer with ZIP6, and this pair has been linked to zinc-dependent epithelial-to-mesenchymal transition and entry into mitosis, while ZIP10 is also needed for normal B-cell development and function.
| Target | Zinc transporter ZIP10 (gene SLC39A10; UniProt Q9ULF5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 94.1 kDa |
| Observed MW | 95–100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Caco-2 cells, human A549 cells, human SiHa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human brest cancer tissue, human thyroid cancer tissue, mouse brain tissue tiss, rat brain tissue tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with confirmed staining (ICC/IF): human thyroid cancer tissue, mouse brain tissue, rat brain tissue.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Recombinant human SLC39A10 fragment (His29–His379), expressed in E. coli. Sequence identity with the mouse ortholog: 77.9%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places zinc transporter ZIP10 in the cell membrane and apical cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
