| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ferroportin; Ferroportin-1; Iron-regulated transporter 1; Solute carrier family 40 member 1; SLC40A1; FPN; FPN1; IREG1; SLC11A3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLC40A1 recombinant protein (Position: M1-V571). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ferroportin (gene SLC40A1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 62.5 kDa, and the supplier reports an observed band at about 70 kDa.
Ferroportin (SLC40A1) is the only known cellular iron exporter in vertebrates. It moves iron out of enterocytes, macrophages and hepatocytes into the blood, and the hormone hepcidin controls systemic iron by binding ferroportin and causing its internalization and degradation; mutations cause ferroportin disease, a form of hemochromatosis.
| Target | Ferroportin (gene SLC40A1; UniProt Q9NP59, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 62.5 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, rat liver tissue, mouse spleen tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human larynx squamous cells carcinoma tissue, human liver cancer tissue, human ovarian serous adenocarcinoma tissue, human prostate adenocarcinoma tissue, human rectum adenocarcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human ovarian cancer tissue.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human SLC40A1 fragment (Met1–Val571), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ferroportin in the cell membrane and basolateral cell membrane. Tissue expression noted by UniProt (human): Detected in erythrocytes (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hangjie Fu et al. (2025) Ellagic acid alleviates iron overload-induced liver damage by mitigating ferroptosis through modulation of the TGFβ/Smad signaling pathway. Food Research International. 10.1016/j.foodres.2025.116590
Chuan-zhi Zhao et al. (2024) Exploring the mechanism of Ling-Gui-Zhu-Gan decoction in metabolic cardiomyopathy via inhibiting ferroptosis. Phytomedicine. 10.1016/j.phymed.2024.156234
Xiaosu Yuan et al. (2025) p53 mediates iron overload and ferroptosis via transcriptional regulation of TfR1 in PM2.5-exposed cardiomyocytes. Journal of Molecular and Cellular Cardiology. 10.1016/j.yjmcc.2025.10.007
Hui Hui et al. (2025) Muscone attenuates myocardial infarction by regulating ferroptosis of cardiomyocytes through Nrf2/system Xc-/GPX4 signaling pathway. International Immunopharmacology. 10.1016/j.intimp.2025.115270
Shi Yue et al. (2023) Ferroptosis is involved in focal segmental glomerulosclerosis in rats. Scientific Reports. 10.1038/s41598-023-49697-8
Wenzhi Liu et al. (2025) Astragalus polysaccharide hinders cervical cancer immune escape by targeting NR3C2 and activating SLC40A1. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2025.168123
Li Bo et al. (2026) Cyclopeptide heterophyllin from Pseudostellaria heterophylla alleviates neuroinflammation: a study integrating molecular dynamics, inflammatory pathway analysis, and in vivo validation. Food Science and Biotechnology. 10.1007/s10068-026-02223-8
Fan Yang et al. (2025) Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells. Experimental Cell Research. 10.1016/j.yexcr.2025.114848
Xiao Xiao et al. (2025) Nuclear factor E2 related factor 2 may alleviate liver injury by regulating iron overload and lipid peroxidation induced by chronic fluorosis. Journal of Trace Elements in Medicine and Biology. 10.1016/j.jtemb.2025.127775
Sun Weichu et al. (2024) Sulforaphane inhibits multiple myeloma cell-induced osteoclast differentiation and macrophage proliferation by elevating ferroportin1. Cancer Chemotherapy and Pharmacology. 10.1007/s00280-024-04736-7
