| Field | Specification |
|---|---|
| Target | |
| Alternative names | Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4; SLC5A4; SAAT1; SGLT3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SLC5A4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets probable glucose sensor protein SLC5A4 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 72.5 kDa, and the supplier reports an observed band at about 72 kDa.
SLC5A4 (SGLT3) belongs to the sodium/glucose cotransporter family but does not transport glucose. Instead it is thought to act as a glucose sensor, producing membrane depolarization when D-glucose binds.
| Target | Probable glucose sensor protein SLC5A4 (gene SLC5A4; UniProt Q9NY91, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 72.5 kDa |
| Observed MW | 72 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human SH-SY5Y cells, human U2OS cells, human 293T cells, human RT4 cells, rat testis tissue, rat liver tissue, mouse testis tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human SLC5A4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places probable glucose sensor protein SLC5A4 in the cell membrane. Tissue expression noted by UniProt (human): Expressed in skeletal muscle, where it may localize to the neuromuscular junction (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
