| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium/hydrogen exchanger 1; APNH; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; NHE-1; Solute carrier family 9 member 1; SLC9A1; APNH1; NHE1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Sodium / Hydrogen Exchanger 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20S20 is a rabbit monoclonal antibody that recognizes sodium/hydrogen exchanger 1 (gene SLC9A1) in human samples and is validated for Western blot. The predicted molecular weight is 90.8 kDa, and the supplier reports an observed band at about 130 kDa.
NHE1 (SLC9A1) is a ubiquitous plasma membrane Na+/H+ exchanger that expels protons in exchange for sodium, protecting cells from acidification. By controlling intracellular pH and cell volume, and by anchoring signaling complexes, it influences cell growth, migration and survival.
| Target | Sodium/hydrogen exchanger 1 (gene SLC9A1; UniProt P19634, human) |
|---|---|
| Clone | 20S20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 90.8 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): MOLT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human sodium / Hydrogen Exchanger 1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places sodium/hydrogen exchanger 1 in the cell membrane and basolateral cell membrane. Tissue expression noted by UniProt (human): Kidney and intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
