Anti-Slime mold patA Antibody

SKU:BHA21011112
Research Validated
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Boster Bio
Boster Bio
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Overview
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Anti-patA antibody (Rabbit polyclonal, Rabbit IgG). Reactivity: Slime mold. Recognizes calcium-transporting ATPase PAT1. Applications are not specified by the supplier; validate the antibody in the intended assay before use.
Target patA
Host Rabbit
Reactivity Slime mold
Isotype Rabbit IgG
Application(s) Western blot, IHC, ELISA, Flow cytometry
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Options selector
Catalog no. Size Conjugation
DZ41342 200 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 200 uL/vial
    • Conjugation (12): Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647, Carrier Free

    Variant-specific details:
    • 200 uL/vial + Unconjugated; 200 uL/vial + Carrier Free
      Storage: At -20℃ for one year, at 4℃ for one month. Avoid repeated freezing and thawing.
      Form: Liquid
      Contents: Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
    • 200 uL/vial + Biotin; 200 uL/vial + Cy3; 200 uL/vial + Fluoro488; 200 uL/vial + Fluoro550; 200 uL/vial + Fluoro594; 200 uL/vial + FITC; 200 uL/vial + APC; 200 uL/vial + PE; 200 uL/vial + Fluoro647
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Form: Liquid
      Applications: Western blot, IHC, ELISA, Flow cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cells
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 200 uL/vial + HRP
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Form: Liquid
      Applications: Western blot, IHC, ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target patA
Alternative names Calcium-transporting ATPase PAT1; PAT1; patA
Gene ID 8621371
UniProt # P54678
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Slime mold
Applications
  • Western Blot
  • Immunohistochemistry
  • ELISA
  • Flow Cytometry
Conjugate
  • Biotin
  • Cy3
  • Fluoro488
  • Fluoro550
  • Fluoro594
  • FITC
  • HRP
  • APC
  • PE
  • Fluoro647
  • Carrier Free
Purification Immunogen affinity purified.
Cellular localization Contractile vacuole membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Concentration 500 μg/ml
Form Liquid
Storage At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Catalog no. (Mfr.) DZ41342
Main SKU BHA21011112
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody detects calcium-transporting ATPase PAT1 (gene patA) in Slime mold samples.

In Dictyostelium discoideum, patA encodes PAT1, a P-type Ca2+-transporting ATPase. It is located on the contractile vacuole system, where it pumps calcium and contributes to Ca2+ homeostasis in this amoeba.

Target Calcium-transporting ATPase PAT1 (gene patA; UniProt P54678, Dictyostelium discoideum)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Slime mold
Form Liquid
Formulation 20 mM PBS; 50% glycerol; 0.02% NaN3
Storage Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Reactivity Notes

The supplier lists reactivity with Slime mold. UniProt places calcium-transporting ATPase PAT1 in the contractile vacuole membrane and cell membrane.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which species does this antibody react with?
A.Validated reactivity: Slime mold. Other species are not listed by the supplier.
Q.What are the host species and clonality?
A.Host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG.
Q.In what form and buffer is the antibody supplied?
A.Liquid; 20 mM PBS; 50% glycerol; 0.02% NaN₃.
Q.How should this antibody be stored?
A.Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Amber D. Ide et al. (2025) Copine A is essential for calcium homeostasis in Dictyostelium. Cell Calcium. 10.1016/j.ceca.2025.103055

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