| Field | Specification |
|---|---|
| Target | |
| Alternative names | SLIT and NTRK-like protein 1; Leucine-rich repeat-containing protein 12; SLITRK1; KIAA1910; LRRC12 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLITRK1 recombinant protein (Position: D22-D696). Human SLITRK1 shares 97.5% amino acid (aa) sequence identity with mouse SLITRK1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets SLIT and NTRK-like protein 1 (gene SLITRK1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 77.7 kDa, and the supplier reports an observed band at about 55 kDa.
SLITRK1 is a neuronal transmembrane protein with leucine-rich repeats related to both Slit and Trk proteins. It promotes the formation of excitatory synapses and the outgrowth of dendrites, and is studied in brain development and neuropsychiatric conditions.
| Target | SLIT and NTRK-like protein 1 (gene SLITRK1; UniProt Q96PX8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 77.7 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U-87MG cells, human U251 cells, human U2OS cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U251 cells.
Immunogen
Recombinant human SLITRK1 fragment (Asp22–Asp696), expressed in E. coli. Sequence identity with the mouse ortholog: 97.5%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes SLIT and NTRK-like protein 1 as secreted and also located in the membrane and synapse. Tissue expression noted by UniProt (human): Expressed predominantly in the frontal lobe of the cerebral cortex of the brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
