| Field | Specification |
|---|---|
| Target | |
| Alternative names | Diablo homolog, mitochondrial; IAP-binding protein with low pI;Second mitochondria-derived activator of caspase;Smac;DIABLO;SMAC; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Smac/Diablo |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FGG-4 is a rabbit monoclonal antibody that recognizes diablo IAP-binding mitochondrial protein in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 27.1 kDa, and the supplier reports an observed band at about 21 kDa.
DIABLO (also called Smac) is a mitochondrial protein released into the cytosol during apoptosis. It binds inhibitor of apoptosis proteins such as XIAP and blocks their ability to restrain caspases, thereby promoting caspase activation downstream of cytochrome c. Smac mimetic compounds are based on its IAP-binding motif, and DIABLO is studied in apoptosis research.
| Target | Diablo IAP-binding mitochondrial protein (gene DIABLO; UniProt Q9NR28, human) |
|---|---|
| Clone | FGG-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 27.1 kDa |
| Observed MW | 21 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human smac/Diablo.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places diablo IAP-binding mitochondrial protein in the mitochondrion and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed with highest expression in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chang Xiaopan et al. (2024) Inhibition of Notch3/Hey1 ameliorates peribiliary hypoxia by preventing hypertrophic hepatic arteriopathy in biliary atresia progression. Histochemistry and Cell Biology. 10.1007/s00418-024-02278-w
