| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mothers against decapentaplegic homolog 4;MAD homolog 4;Mothers against DPP homolog 4;Deletion target in pancreatic carcinoma 4;SMAD family member 4;SMAD 4;Smad4;hSMAD4;SMAD4;DPC4, MADH4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Smad4, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes SMAD family member 4 (gene SMAD4) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 60.4 kDa, and the supplier reports an observed band at about 60 kDa.
SMAD4 is the common partner SMAD that combines with receptor-activated SMADs in both TGF-β and BMP signaling. The resulting complexes enter the nucleus to control gene transcription. SMAD4 is a well-known tumor suppressor, frequently lost in pancreatic and colorectal cancers.
| Target | SMAD family member 4 (gene SMAD4; UniProt Q13485, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 60.4 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, U87 cells, human placenta tissue, HT1080 cells, HeLa cells, NEURO cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human smad4. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places SMAD family member 4 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Minghong Dong et al. (2026) Integrated serum pharmacochemistry, network pharmacology and transcriptomics reveals the mechanism of Periploca forrestii on rheumatoid arthritis bone erosion. Journal of Ethnopharmacology. 10.1016/j.jep.2026.121197
Liyun Gao et al. (2019) 2,3,7,8-Tetrachlorodibenzo-p-dioxin and TGFβ3-Mediated Mouse Embryonic Palatal Mesenchymal Cells. Dose-Response. 10.1177/1559325818786822
Gao Liyun et al. (2018) 2,3,7,8-Tetrachlorodibenzo-p-Dioxin and TGF-β3 Mediated-Mouse Embryonic Palatal Mesenchymal Cells. Dose-Response. 10.1177/1559325818810637
Yuanyuan Ji et al. (2016) Sodium humate accelerates cutaneous wound healing by activating TGF-β/Smads signaling pathway in rats. Acta Pharmaceutica Sinica B. 10.1016/j.apsb.2016.01.009
