| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5;SMAD5;MADH5; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Smad5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BDB-19 is a rabbit monoclonal antibody that recognizes SMAD family member 5 (gene SMAD5) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 52.3 kDa, and the supplier reports an observed band at about 52 kDa.
SMAD5 is a receptor-regulated SMAD in the BMP pathway. When phosphorylated by BMP type I receptors it binds SMAD4 and regulates gene transcription in development, cell differentiation and angiogenesis; the unphosphorylated protein has also been linked to regulation of glycolysis in the cytoplasm.
| Target | SMAD family member 5 (gene SMAD5; UniProt Q99717, human) |
|---|---|
| Clone | BDB-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 52.3 kDa |
| Observed MW | 52 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human Caco-2 cells, rat C6 cells, rat PC-12 cells, mouse Hepa1–6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human stomach cancer tissue, human Hodgkin's lymphoma tissue, human glioblastoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human smad5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places SMAD family member 5 in the cytoplasm, nucleus and mitochondrion. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
