| Field | Specification |
|---|---|
| Target | |
| Alternative names | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1; 60 kDa BRG-1/Brm-associated factor subunit A; BRG1-associated factor 60A; BAF60A; SWI/SNF complex 60 kDa subunit; SMARCD1 |
| UniProt # | |
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| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SMARCD, identical to the related mouse sequences. |
| Molecular weight | |
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| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
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Product Overview
This rabbit polyclonal antibody recognizes SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 (gene SMARCD1) in human samples and is validated for Western blot. The predicted molecular weight is 58.2 kDa, and the supplier reports an observed band at about 58 kDa.
BAF60A (SMARCD1) is a subunit of SWI/SNF (BAF) chromatin remodeling complexes. It helps link the complex to transcription factors and nuclear receptors, contributing to gene activation and repression during development.
| Target | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 (gene SMARCD1; UniProt Q96GM5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 58.2 kDa |
| Observed MW | 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human PANC-1 cells, human Jurkat cells, human MOLT-4 cells, human Caco-2 cells, human SH-SY5Y cells, human Daudi cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human SMARCD. The peptide sequence is conserved in mouse.
Reactivity Notes
The supplier lists reactivity with human. UniProt places SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 in the nucleus. Tissue expression noted by UniProt (human): Expressed in all tissues tested, including brain, heart, kidney, liver, lung, muscle, pancreas and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
