| Field | Specification |
|---|---|
| Target | |
| Alternative names | Structural maintenance of chromosomes protein 1A;SMC protein 1A;SMC-1-alpha;SMC-1A;Sb1.8;SMC1A;DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SMC1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GHE-19 is a rabbit monoclonal antibody that recognizes structural maintenance of chromosomes protein 1A (gene SMC1A) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 143.2 kDa, and the supplier reports an observed band at about 143 kDa.
SMC1A is a core subunit of cohesin, a ring-shaped complex that holds sister chromatids together from DNA replication until anaphase. Cohesin also helps shape chromatin loops and supports DNA repair, and SMC1A is phosphorylated by ATM after DNA damage. Mutations cause a form of Cornelia de Lange syndrome.
| Target | Structural maintenance of chromosomes protein 1A (gene SMC1A; UniProt Q14683, human) |
|---|---|
| Clone | GHE-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 143.2 kDa |
| Observed MW | 143 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells, Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): mouse colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human SMC1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places structural maintenance of chromosomes protein 1A in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
