| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ras-related protein Rab-4A;RAB4A;RAB4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human SMCHD1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-SMCHD1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23S66; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-SMCHD1 Rabbit Monoclonal Antibody catalog # M04751. Tested in WB, IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: SMCHD1 (Ras-related protein Rab-4A).
- Antibody format: Monoclonal; clone 23S66; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
SMCHD1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Protein transport. Probably involved in vesicular traffic (By similarity). . Reported cellular localization context: Membrane; Peripheral membrane protein. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1. Tissue expression notes (as provided): Widely expressed, including brain and spinal cord. .
Research relevance and current trends
- Research context keywords from the source record include: Epigenetics and Nuclear Signaling,Transcription,2339,Co-Factors,Chromatin Remodeling.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate SMCHD1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect SMCHD1 expression by Western blot in cell or tissue lysates, Detect SMCHD1 in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 226 kDa; calculated MW: 24390 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 226 kDa
- Cellular localization (provided): Membrane; Peripheral membrane protein. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.
- Tissue details (provided): Widely expressed, including brain and spinal cord. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
