| Field | Specification |
|---|---|
| Target | |
| Alternative names | Structural maintenance of chromosomes flexible hinge domain-containing protein 1; SMC hinge domain-containing protein 1; SMCHD1; KIAA0650 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SMCHD1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23S66 is a rabbit monoclonal antibody that recognizes structural maintenance of chromosomes flexible hinge domain-containing protein 1 (gene SMCHD1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 226.4 kDa, and the supplier reports an observed band at about 226 kDa.
SMCHD1 is a non-canonical member of the SMC protein family that promotes epigenetic silencing by shaping chromatin architecture. It is important for X chromosome inactivation and for silencing of certain autosomal genes, and its mutation is linked to facioscapulohumeral muscular dystrophy type 2.
| Target | Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (gene SMCHD1; UniProt A6NHR9, human) |
|---|---|
| Clone | 23S66 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 226.4 kDa |
| Observed MW | 226 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human U2OS cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human SMCHD1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places structural maintenance of chromosomes flexible hinge domain-containing protein 1 in the chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
