| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase SMG1; SMG-1; hSMG-1; Lambda/iota protein kinase C-interacting protein; Lambda-interacting protein; Nonsense mediated mRNA decay-associated PI3K-related kinase SMG1; SMG1; ATX; KIAA0421; LIP |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Smg1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25S75 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase SMG1 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 410.5 kDa, and the supplier reports an observed band at about 410 kDa.
SMG1 is a PI3K-related protein kinase with two main roles. In nonsense-mediated mRNA decay (NMD) it phosphorylates UPF1 to trigger degradation of mRNAs with premature stop codons, and it also takes part in the cellular response to genotoxic stress.
| Target | Serine/threonine-protein kinase SMG1 (gene SMG1; UniProt Q96Q15, human) |
|---|---|
| Clone | 25S75 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 410.5 kDa |
| Observed MW | 410 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Saos2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human smg1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase SMG1 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed, with highest level in heart and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
