| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sphingomyelin phosphodiesterase 3; Neutral sphingomyelinase 2; nSMase-2; nSMase2; Neutral sphingomyelinase II; SMPD3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SMPD3 recombinant protein (Position: F184-D553). Human SMPD3 shares 88.9% amino acid (aa) sequence identity with both mouse and rat SMPD3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes sphingomyelin phosphodiesterase 3 (gene SMPD3) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 71.1 kDa, and the supplier reports an observed band at about 75 kDa.
Neutral sphingomyelinase 2 (SMPD3) hydrolyzes sphingomyelin to ceramide and phosphocholine at cell membranes. Ceramide from this enzyme influences cell growth arrest and apoptosis, and SMPD3 is needed for normal bone and dentin mineralization; it is also studied in exosome formation.
| Target | Sphingomyelin phosphodiesterase 3 (gene SMPD3; UniProt Q9NY59, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 71.1 kDa |
| Observed MW | 75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human SMPD3 fragment (Phe184–Asp553), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 88.9%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places sphingomyelin phosphodiesterase 3 in the Golgi apparatus membrane and cell membrane. Tissue expression noted by UniProt (human): Predominantly expressed in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
