Anti-SNIP1 Antibody Picoband®

Overview

Anti-SNIP1 Antibody Picoband® is an antibody for SNIP1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: SNIP1 (tetraspanin 12); UniProt: Q8TAD8
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 46 kDa, calculated 39411 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-SNIP1 Antibody Picoband® catalog # A05476-1.

Biological background

Biological context: Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1) (By similarity). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity.

Expression and localization notes: cellular localization: Cell membrane; Multi-pass membrane protein., tissue context: Expressed in testis, in Sertoli cells, ejaculated spermatozoa and in seminal fluid (at protein level)..

Common research applications

• Western blotting (WB): Compare SNIP1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of SNIP1 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify SNIP1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Smad nuclear-interacting protein 1 is a protein that in humans is encoded by the SNIP1 gene. This gene encodes a protein that contains a coiled-coil motif and C-terminal forkhead-associated (FHA) domain. The encoded protein functions as a transcriptional coactivator that increases c-Myc activity and inhibits transforming growth factor beta (TGF-beta) and nuclear factor kappa-B (NF-kB) signaling. The encoded protein also regulates the stability of cyclin D1 mRNA, and may play a role in cell proliferation and cancer progression. Mutations in this gene are a cause of psychomotor retardation, epilepsy, and craniofacial dysmorphism (PMRED).
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane; Multi-pass membrane protein.
• Tissue details: Expressed in testis, in Sertoli cells, ejaculated spermatozoa and in seminal fluid (at protein level).
• Research category: Alzheimer's Disease,Cell Biology,Metalloprotease,Neurodegenerative Disease,Neurology Process,Neuroscience,Proteolysis/Ubiquitin,Proteolytic Enzymes,Sensory System,Visual System

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.