| Field | Specification |
|---|---|
| Target | |
| Alternative names | U5 small nuclear ribonucleoprotein 200 kDa helicase; Activating signal cointegrator 1 complex subunit 3-like 1; BRR2 homolog; U5 snRNP-specific 200 kDa protein; U5-200KD; SNRNP200; ASCC3L1; BRR2; HELIC2; KIAA0788 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SNRNP200 recombinant protein (Position: K557-A2129). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets U5 small nuclear ribonucleoprotein 200 kDa helicase (gene SNRNP200) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 244.5 kDa, and the supplier reports an observed band at about 245 kDa.
SNRNP200 (BRR2) is the RNA helicase of the U5 snRNP that unwinds the U4/U6 RNA duplex, a key step in activating the spliceosome. It is a core component throughout the splicing cycle, and mutations cause a form of retinitis pigmentosa.
| Target | U5 small nuclear ribonucleoprotein 200 kDa helicase (gene SNRNP200; UniProt O75643, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 244.5 kDa |
| Observed MW | 245 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human diffuse large B cells lymphoma tissue, human duodenal papilla adenocarcinoma tissue, human endometrioid adenocarcinoma tissue, human glioblastoma tissue, human larynx squamous cells carcinoma tissue, human liver cancer tissue, human lung adenocarcinoma tissue, human placenta tissue, human prostate adenocarcinoma tissue, human spleen tissue, human testicular seminoma tissue, mouse brain tissue, mouse kidney tissue, rat brain tissue, mouse colon tissue, rat colon tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Samples with confirmed staining (ICC/IF): human intestinal cancer tissue.
Samples with a confirmed signal (flow cytometry): A431 cells, ANA-1 cells.
Immunogen
Recombinant human SNRNP200 fragment (Lys557–Ala2129), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places U5 small nuclear ribonucleoprotein 200 kDa helicase in the nucleus. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
