| Field | Specification |
|---|---|
| Target | |
| Alternative names | Small nuclear ribonucleoprotein Sm D2; Sm-D2; snRNP core protein D2; SNRPD2; SNRPD1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SNRPD2 recombinant protein (Position: M1-K118). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes small nuclear ribonucleoprotein Sm D2 (gene SNRPD2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 13.5 kDa, and the supplier reports an observed band at about 16 kDa.
SmD2 (SNRPD2) is one of the seven Sm proteins that form a ring on small nuclear RNAs, creating the core of the U1, U2, U4 and U5 snRNPs. It is therefore a core component of the spliceosome needed for pre-mRNA splicing.
| Target | Small nuclear ribonucleoprotein Sm D2 (gene SNRPD2; UniProt P62316, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 13.5 kDa |
| Observed MW | 16 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human HL-60 cells, human THP-1 cells, rat testis tissue, mouse testis tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): diffuse large B-cells lymphoma of human intestine tissue, human appendix adenocarcinoma tissue, human breast cancer tissue, human colon adenocarcinoma tissue, human endometrioid adenocarcinoma tissue, human glioblastoma tissue, human liver cancer tissue, human prostate adenocarcinoma tissue, human right renal oncocytoma tissue, human thyroid papillary carcinoma tissue, human urothelial carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): SIHA cells.
Samples with confirmed staining (ICC/IF): human squamous carcinoma tissue, human breast cancer tissue.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human SNRPD2 fragment (Met1–Lys118), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places small nuclear ribonucleoprotein Sm D2 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
