| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sorting nexin-5; SNX5 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SNX5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30S11 is a rabbit monoclonal antibody that recognizes sorting nexin-5 (gene SNX5) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 46.8 kDa, and the supplier reports an observed band at about 47 kDa.
Sorting nexin-5 (SNX5) is a PX-BAR protein that binds phosphoinositide-containing endosomal membranes. As part of the SNX-BAR retromer subcomplex it bends membranes into tubular carriers that return cargo receptors from endosomes to the trans-Golgi network or to the plasma membrane, and it is widely studied in endosomal sorting, receptor recycling and pathogen trafficking.
| Target | Sorting nexin-5 (gene SNX5; UniProt Q9Y5X3, human) |
|---|---|
| Clone | 30S11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.8 kDa |
| Observed MW | 47 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SNX5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sorting nexin-5 in the endosome, early endosome and early endosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
