| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sterol O-acyltransferase 1; Acyl-coenzyme A:cholesterol acyltransferase 1; ACAT-1; Cholesterol acyltransferase 1; SOAT1; ACACT; ACACT1; ACAT; ACAT1; SOAT; STAT |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SOAT1 recombinant protein (Position: R41-Y548). Human SOAT1 shares 88.2% and 87.4% amino acid (aa) sequence identity with mouse and rat SOAT1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sterol O-acyltransferase 1 (gene SOAT1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 64.7 kDa, and the supplier reports an observed band at about 75 kDa.
Sterol O-acyltransferase 1 (SOAT1, also called ACAT1) is an endoplasmic reticulum enzyme that joins fatty acyl-CoA, preferably oleoyl-CoA, to cholesterol to form cholesteryl esters. These esters are stored in lipid droplets, so SOAT1 helps control free cholesterol levels in membranes and contributes to lipoprotein assembly and dietary cholesterol handling; it is studied in lipid metabolism and foam cell biology.
| Target | Sterol O-acyltransferase 1 (gene SOAT1; UniProt P35610, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 64.7 kDa |
| Observed MW | 75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human HUH-7 cells, human THP-1 cells, human HepG2 cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human prostatic cancer tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): PC-3 cells.
Samples with a confirmed signal (flow cytometry): Caco-2 cells, THP-1 cells.
Immunogen
Recombinant human SOAT1 fragment (Arg41–Tyr548), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 88.2% and 87.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sterol O-acyltransferase 1 in the endoplasmic reticulum membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fu Rongrong et al. (2024) SOAT1 regulates cholesterol metabolism to induce EMT in hepatocellular carcinoma. Cell Death & Disease. 10.1038/s41419-024-06711-9
