| Field | Specification |
|---|---|
| Target | |
| Alternative names | Superoxide dismutase [Cu-Zn];1.15.1.1;Superoxide dismutase 1;hSod1;SOD1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SOD1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOEI-19 is a rabbit monoclonal antibody that recognizes superoxide dismutase (gene SOD1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 15.9 kDa, and the supplier reports an observed band at about 16–18 kDa.
Superoxide dismutase 1 (SOD1) is a copper-zinc enzyme found mainly in the cytosol that converts superoxide radicals into hydrogen peroxide and oxygen, forming a first line of antioxidant defense. It can also help detoxify hydrogen sulfide. Mutations in SOD1 are a well-established cause of familial amyotrophic lateral sclerosis, so it is widely studied in oxidative stress and neurodegeneration.
| Target | Superoxide dismutase (gene SOD1; UniProt P00441, human) |
|---|---|
| Clone | AOEI-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 15.9 kDa |
| Observed MW | 16–18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human SIHA cells, human HEL cells, human HepG2 cells, human MCF-7 cells, human Jurkat cells, human HeLa cells, human PC-3 cells, rat brain tissue, rat C6 cells, rat liver muscle tissue, mouse brain tissue, mouse liver tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human SOD1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places superoxide dismutase in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Cao Qianlin et al. (2017) A single low dose of Fe ions can cause long-term biological responses in NL20 human bronchial epithelial cells. Radiation and Environmental Biophysics. 10.1007/s00411-017-0719-0
