| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Sodium/potassium-transporting ATPase subunit alpha-1;Na (+)/K (+) ATPase alpha-1 subunit;3.6.3.9;Sodium pump subunit alpha-1;ATP1A1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Sodium Potassium ATPase |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-ATP1A1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AID-1; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody catalog # M00956. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: ATP1A1 (Sodium/potassium-transporting ATPase subunit alpha-1).
- Antibody format: Monoclonal; clone AID-1; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ATP1A1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Reported cellular localization context: Cell membrane; Multi-pass membrane protein. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue expression notes (as provided): Expressed in the excretory system, thyroid gland and Wilms tumors.
Research relevance and current trends
- Research context keywords from the source record include: ATPases,Cancer,Cancer Metabolism,Cell Type Marker,Metabolism,Neurogenesis,Neurology Process,Neuron Marker,Neuroscience,Plasma Membrane,Response To Hypoxia,Signal Transduction,Subcellular Markers,Synapse Marker,Tags & Cell Markers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate ATP1A1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ATP1A1 expression by Western blot in cell or tissue lysates, Detect ATP1A1 in FFPE tissue sections by immunohistochemistry, Localize ATP1A1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify ATP1A1-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 28 kDa; calculated MW: 112896 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 28 kDa
- Cellular localization (provided): Cell membrane; Multi-pass membrane protein. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Tissue details (provided): Expressed in the excretory system, thyroid gland and Wilms tumors.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
