| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium/potassium-transporting ATPase subunit alpha-1;Na (+)/K (+) ATPase alpha-1 subunit;3.6.3.9;Sodium pump subunit alpha-1;ATP1A1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Sodium Potassium ATPase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AID-1 is a rabbit monoclonal antibody that recognizes sodium/potassium-transporting ATPase subunit alpha-1 (gene ATP1A1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 112.9 kDa, and the supplier reports an observed band at about 100 kDa.
ATP1A1 is the ubiquitous catalytic α1 subunit of the Na+/K+-ATPase. Using ATP, the pump exports 3 Na+ and imports 2 K+ per cycle, maintaining the gradients needed for membrane potential and secondary transport, and it is the binding site for cardiac glycosides such as ouabain.
| Target | Sodium/potassium-transporting ATPase subunit alpha-1 (gene ATP1A1; UniProt P05023, human) |
|---|---|
| Clone | AID-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 112.9 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:5, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): rat cerebral cortex tissue, rat heart tissue, human cervical cancer tissue, human thyroid cancer tissue, mouse skeletal muscle - gastrocnemius tissue, human thyroid carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human sodium Potassium ATPase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sodium/potassium-transporting ATPase subunit alpha-1 in the cell membrane, basolateral cell membrane, cell projection and melanosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Pi Zhao et al. (2026) A self-amplifying cuproptosis nanomedicine to overcome immunosuppression by blocking tumor-derived exosomes for enhancing lung cancer immunotherapy. Journal of Controlled Release. 10.1016/j.jconrel.2025.114593
