| Field | Specification |
|---|---|
| Target | |
| Alternative names | Somatostatin receptor type 2;SS-2-R;SS2-R;SS2R;SRIF-1;SSTR2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Somatostatin Receptor 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CIF-19 is a rabbit monoclonal antibody that recognizes somatostatin receptor type 2 (gene SSTR2) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 41.3 kDa, and the supplier reports an observed band at about 41 kDa.
Somatostatin receptor 2 (SSTR2) is a G protein-coupled receptor for somatostatin-14 and -28 that reduces cAMP production and limits calcium entry through voltage-gated channels. It is the dominant somatostatin receptor on pancreatic alpha and beta cells, where it restrains hormone release, and it also has antiproliferative effects, so it is widely studied in neuroendocrine biology and tumors.
| Target | Somatostatin receptor type 2 (gene SSTR2; UniProt P30874, human) |
|---|---|
| Clone | CIF-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.3 kDa |
| Observed MW | 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human K562 cells, human SH-SY5Y cells, human Jurkat cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human somatostatin Receptor 2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places somatostatin receptor type 2 in the cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Expressed in both pancreatic alpha- and beta-cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Sharma Rupali et al. (2023) Upregulation of Somatostatin Receptor Type 2 Improves 177Lu-DOTATATE Therapy in Receptor-Deficient Pancreatic Neuroendocrine Tumor Model. Molecular Cancer Therapeutics. 10.1158/1535-7163.MCT-22-0798
Yan Xie et al. (2011) SOM230 combined with celecoxib prolongs the survival in nude mice with HepG-2 xenografts. Cancer Biology & Therapy. 10.4161/cbt.12.1.15730
