| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Sonic hedgehog protein;SHH;HHG-1;Sonic hedgehog protein N-product;Sonic hedgehog protein C-product;SHH; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Sonic Hedgehog |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-SHH antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ABE-19; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-Sonic Hedgehog SHH Rabbit Monoclonal Antibody catalog # M00058. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: SHH (Sonic hedgehog protein).
- Antibody format: Monoclonal; clone ABE-19; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
SHH (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity). . Reported cellular localization context: Sonic hedgehog protein C-product: Secreted, extracellular space . The C-terminal peptide diffuses from the cell. . Tissue expression notes (as provided): Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cancer Metabolism,Developmental Biology,Developmental Families,Domain Families,Embryogenesis,Embryonic Stem Cells,Epigenetics and Nuclear Signaling,Hypoxia,Intracellular,Metabolism,Metabolism Processes,Neural Stem Cells,Oncoproteins/Suppressors,Organogenesis,Pathways and Processes,Response To Hypoxia,Secreted,Signaling Pathways,Stem Cells,Surface Molecules,Transcription,Tumor Suppressors.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate SHH antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect SHH expression by Western blot in cell or tissue lysates, Detect SHH in FFPE tissue sections by immunohistochemistry, Localize SHH by immunofluorescence/immunocytochemistry in cultured cells, Quantify SHH-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 50 kDa; calculated MW: 49607 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 50 kDa
- Cellular localization (provided): Sonic hedgehog protein C-product: Secreted, extracellular space . The C-terminal peptide diffuses from the cell. .
- Tissue details (provided): Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
