| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor SOX-10;SOX10; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SOX10 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GIB-19 is a rabbit monoclonal antibody that recognizes transcription factor SOX-10 (gene SOX10) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 49.9 kDa, and the supplier reports an observed band at about 60–65 kDa.
Transcription factor SOX-10 (SOX10) is an HMG-box transcription factor central to neural crest derivatives and glial cells. It drives the myelination program in oligodendrocytes, including induction of MYRF and myelin genes, and together with PAX3 activates MITF in melanocytes, so SOX10 is a widely used marker for oligodendrocytes, Schwann cells and melanocytic cells.
| Target | Transcription factor SOX-10 (gene SOX10; UniProt P56693, human) |
|---|---|
| Clone | GIB-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 49.9 kDa |
| Observed MW | 60–65 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human A375 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (ICC/IF): HT-29 cells, A-375 cells.
Immunogen
Synthetic peptide based on the sequence of human SOX10.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor SOX-10 in the cytoplasm, nucleus and mitochondrion outer membrane. Tissue expression noted by UniProt (human): Expressed in fetal brain and in adult brain, heart, small intestine and colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xinyuan Hu et al. (2018) Identification and differentiation therapy strategy of pterygium in vitro. American Journal of Translational Research. PMID: 30210698
Yuan Li et al. (2022) Establishment of an astrocyte-like cell line from the brain of tilapia (Oreochromis niloticus) for virus pathogenesis and a vitro model of the blood–brain barrier. Journal of Fish Diseases. 10.1111/jfd.13674
