| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor SOX-2;SOX2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SOX2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BIO-19 is a rabbit monoclonal antibody that recognizes transcription factor SOX-2 (gene SOX2) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 34.3 kDa, and the supplier reports an observed band at about 34 kDa.
Transcription factor SOX-2 (SOX2) is a SoxB1 HMG-box factor that partners with OCT4 to control genes needed for early embryonic development and embryonic stem cell pluripotency. It is one of the classic reprogramming factors and also keeps neural progenitors self-renewing, so SOX2 is a widely used marker of pluripotent and neural stem cells.
| Target | Transcription factor SOX-2 (gene SOX2; UniProt P48431, human) |
|---|---|
| Clone | BIO-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.3 kDa |
| Observed MW | 34 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human cervix carcinoma tissue, human cervical cancer tissue, human tonsilitis tissue, mouse kidney tissue, mouse stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human cervical cancer tissue, human testis cancer tissue.
Immunogen
Synthetic peptide based on the sequence of human SOX2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor SOX-2 in the nucleus speckle, cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huan Gong et al. (2025) Carboxymethyl chitosan hydrogel synergistically regulates neural stem cells with ultrasound for the treatment of traumatic brain injury. Materials Today Advances. 10.1016/j.mtadv.2025.100639
Liang Yao et al. (2026) KCTD15 Enhances Stem Cell‐Like Properties and Promotes Triple‐Negative Breast Cancer Progression Through KLF4/β‐Catenin Signaling. FASEB Journal. 10.1096/fj.202503381R
Peiyu Sun et al. (2025) Ginsenoside Rg1 Promotes the Survival, Proliferation, and Differentiation of Senescent Neural Stem Cells Induced by D-galactose. Actas Espanolas de Psiquiatria. 10.62641/aep.v53i1.1812
