| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor SOX-7; SOX7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SOX7 recombinant protein (Position: K57-D343). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transcription factor SOX-7 (gene SOX7) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 42.2 kDa, and the supplier reports an observed band at about 43 kDa.
Transcription factor SOX-7 (SOX7) is a SoxF group HMG-box factor expressed in endothelial and hemogenic endothelial cells. It activates VE-cadherin (CDH5) and other endothelial genes, holds back differentiation of blood precursors, and can dampen Wnt/β-catenin-driven transcription, so it is studied in vascular and blood development.
| Target | Transcription factor SOX-7 (gene SOX7; UniProt Q9BT81, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 42.2 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Colo320 cells, human PC-3 cells, human A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human SOX7 fragment (Lys57–Asp343), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transcription factor SOX-7 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed in adult and fetal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
