| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor SOX-9; SOX9 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SOX9, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes transcription factor SOX-9 (gene SOX9) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 56.1 kDa, and the supplier reports an observed band at about 70 kDa.
Transcription factor SOX-9 (SOX9) is an HMG-box factor that is essential for chondrocyte differentiation and skeletal development, activating cartilage matrix genes such as COL2A1 and aggrecan. It is also required for Sertoli cell development in male sex determination and for many other organs, making SOX9 a widely used marker in cartilage, testis and stem cell research.
| Target | Transcription factor SOX-9 (gene SOX9; UniProt P48436, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 56.1 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, mouse testis tissue, rat lung tissue, rat testis tissue, human intestinal cancer tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the middle region of human SOX9. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor SOX-9 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
He Zhiyi et al. (2024) Remodeling of the pro-inflammatory microenvironment in osteoarthritis via hydrogel-based photothermal therapy. Advanced Composites and Hybrid Materials. 10.1007/s42114-024-00835-4
Wan Junlai et al. (2023) Novel strategy of senescence elimination via toxicity-exempted kinome perturbations by nanoliposome-based thermosensitive hydrogel for osteoarthritis therapy. Advanced Composites and Hybrid Materials. 10.1007/s42114-023-00673-w
Wenchao Wang et al. (2026) mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via Piezo1-NCOA4-ZBP1 axis. Autophagy. 10.1080/15548627.2026.2677183
Wan Junlai et al. (2023) Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy. Journal of Nanobiotechnology. 10.1186/s12951-023-02050-7
Zhiwei Zhang et al. (2023) Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2023.114964
Yuandong Shao et al. (2022) Icariin protects vertebral endplate chondrocytes against apoptosis and degeneration via activating Nrf-2/HO-1 pathway. Frontiers in Pharmacology. 10.3389/fphar.2022.937502
Xingzhi Jing et al. (2024) HIF-2α/TFR1 mediated iron homeostasis disruption aggravates cartilage endplate degeneration through ferroptotic damage and mtDNA release: A new mechanism of intervertebral disc degeneration. Journal of Orthopaedic Translation. 10.1016/j.jot.2024.03.005
Tongmeng Jiang et al. (2018) Intra-hydrogel culture prevents transformation of mesenchymal stem cells induced by monolayer expansion. Biomaterials Science. 10.1039/C8BM00007G
Guihe Yang et al. (2023) Astaxanthin suppresses oxidative stress and calcification in vertebral cartilage endplate via activating Nrf-2/HO-1 signaling pathway. International Immunopharmacology. 10.1016/j.intimp.2023.110159
Zhenkai Ma et al. (2023) Nrf2 protects against cartilage endplate degeneration through inhibiting NCOA4‑mediated ferritinophagy. International Journal of Molecular Medicine. 10.3892/ijmm.2023.5339
