| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor SOX-9;SOX9; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SOX9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DAO-19 is a rabbit monoclonal antibody that recognizes transcription factor SOX-9 (gene SOX9) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 56.1 kDa, and the supplier reports an observed band at about 70 kDa.
Transcription factor SOX-9 (SOX9) is an HMG-box factor that is essential for chondrocyte differentiation and skeletal development, activating cartilage matrix genes such as COL2A1 and aggrecan. It is also required for Sertoli cell development in male sex determination and for many other organs, making SOX9 a widely used marker in cartilage, testis and stem cell research.
| Target | Transcription factor SOX-9 (gene SOX9; UniProt P48436, human) |
|---|---|
| Clone | DAO-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.1 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human Caco-2 cells, human SW620 cells, rat PC-12 cells, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human liver cancer tissue, human prostate cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): SW480 cells.
Immunogen
Synthetic peptide based on the sequence of human SOX9.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor SOX-9 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Juan Yang et al. (2023) Inhibition of p38MAPK signal pathway alleviates radiation-induced testicular damage through improving spermatogenesis. British Journal of Pharmacology. 10.1111/bph.16217
Yang Wu et al. (2016) RETRACTED: Functional quantum dot-siRNA nanoplexes to regulate chondrogenic differentiation of mesenchymal stem cells. Acta Biomaterialia. 10.1016/j.actbio.2016.09.008
Dongyang Li et al. (2026) Cyanidin-3-O-glucoside chloride inhibits cartilage degeneration and inflammation in TMJOA via YAP/NF-κB signaling pathway. Food & Function. 10.1039/D5FO04230E
Jianing Tan et al. (2023) Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration. International Journal of Molecular Medicine. 10.3892/ijmm.2023.5307
Demin Lv et al. (2024) LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts. Molecular Medicine Reports. 10.3892/mmr.2024.13365
Shenghui Liu (2024) AHR regulates liver enlargement and regeneration through the YAP signaling pathway. Heliyon. 10.1016/j.heliyon.2024.e37265
Xinyuan Hu et al. (2018) Identification and differentiation therapy strategy of pterygium in vitro. American Journal of Translational Research. PMID: 30210698
Mengru Zhuang et al. (2018) Reelin regulates male mouse reproductive capacity via the sertoli cells. Journal of Cellular Biochemistry. 10.1002/jcb.26824
Pengzhen Wang et al. (2019) Icariin increases chondrocyte vitality by promoting hypoxia-inducible factor-1α expression and anaerobic glycolysis. Knee. 10.1016/j.knee.2019.09.012
