| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor Sp1; SP1; TSFP1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human SP1 recombinant protein (Position: Q384-A603). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 3C4C3 is a mouse monoclonal antibody that recognizes transcription factor Sp1 in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 80.7 kDa, and the supplier reports an observed band at about 90 kDa.
Transcription factor Sp1 (SP1) is a zinc finger protein that binds GC-rich promoter elements and regulates a very large number of genes involved in growth, apoptosis, differentiation and immune responses. Its activity is tuned by phosphorylation, acetylation, glycosylation and other modifications, and it is one of the most studied general transcription regulators.
| Target | Transcription factor Sp1 (gene SP1; UniProt P08047, human) |
|---|---|
| Clone | 3C4C3 |
| Host / Clonality / Isotype | Mouse / Monoclonal / IgG1 |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 80.7 kDa |
| Observed MW | 90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human Caco-2 cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human glioma tissue, human laryngeal squamous cells carcinoma tissue, human lung cancer tissue, human ovarian cancer tissue, human thyroid cancer tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human SP1 fragment (Gln384–Ala603), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transcription factor Sp1 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Up-regulated in adenocarcinomas of the stomach (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
