| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Transcription factor Sp5;SP5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Sp5, identical to the related mouse sequence. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SP5 (Transcription factor Sp5) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Sp5 Antibody Picoband® catalog # PB9443. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human Sp5, identical to the related mouse sequence.
- Molecular weight context: reported MW: 42 kDa; calculated MW: 41964 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Transcription factor Sp5; Transcription factor Sp5. Sp5 is mapped to 2q31.1. It is a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Elevated expression of Sp5 has been noted in several human tumors including hepatocellular carcinoma, gastric cancer and colon cancer. Functional note: Binds to GC boxes promoters elements. Probable transcriptional activator that has a role in the coordination of changes in transcription required to generate pattern in the developing embryo (By similarity). . Reported localization: Nucleus . Expression/tissue context: Expressed in brain and lymphoblasts. .
Research relevance and current trends
- Domain Families: Researchers commonly examine how SP5 (Transcription factor Sp5) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how SP5 (Transcription factor Sp5) relates to this theme using model systems and orthogonal readouts.
- Signaling Pathways: Researchers commonly examine how SP5 (Transcription factor Sp5) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SP5 (Transcription factor Sp5) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of SP5 (Transcription factor Sp5) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the Sp1 C2H2-type zinc-finger protein family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
