| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor Sp7; Zinc finger protein osterix; SP7; OSX |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Sp7/Osterix recombinant protein (Position: A2-E241). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes transcription factor Sp7 in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 45.0 kDa, and the supplier reports an observed band at about 45 kDa.
Osterix (SP7) is a zinc finger transcriptional activator that is essential for osteoblast differentiation. Acting downstream of RUNX2, it binds GC-rich promoter sequences to switch on bone-forming genes, and it is widely used as a marker of committed osteoblasts in bone development and regeneration studies.
| Target | Transcription factor Sp7 (gene SP7; UniProt Q8TDD2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 45.0 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human U87 cells, human MDA-MB453 cells, human MCF-7 cells, human Jurkat cells, human THP-1 whole cellrat lung tissue, rat thymus tissue, rat skeletal muscle tissue, rat testis tissue, mouse lung tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Sp7/Osterix fragment (Ala2–Glu241), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor Sp7 in the nucleus. Tissue expression noted by UniProt (human): Restricted to bone-derived cell. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Sun Haijian et al. (2026) Targeting N1-methyladenosine modification in osteoblasts through tRNA methyltransferase 10C reverses mitochondrial dysfunction and ameliorates osteoporosis. Signal Transduction and Targeted Therapy. 10.1038/s41392-026-02740-2
Yang Zheng et al. (2023) WISP2 downregulation inhibits the osteogenic differentiation of BMSCs in congenital scoliosis by regulating Wnt/β-catenin pathway. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2023.166783
Wenli Gu et al. (2022) Super-Enhancer-Associated Long Non-Coding RNA LINC01485 Promotes Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells by Regulating MiR-619-5p/RUNX2 Axis. Frontiers in Endocrinology. 10.3389/fendo.2022.846154
Mai Zhaoyi et al. (2023) Long noncoding RNA KCNMA1-AS1 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells by activating the SMAD9 signaling pathway. Biology Direct. 10.1186/s13062-023-00425-2
Liangyan Sun et al. (2023) Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial. Journal of Functional Biomaterials. 10.3390/jfb14020086
Gaoyang Chen et al. (2020) Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head. Bone. 10.1016/j.bone.2020.115258
Xiaobo Wei et al. (2024) Enhancing osteoblast proliferation and bone regeneration by poly (amino acid)/selenium-doped hydroxyapatite. Biomedical Materials. 10.1088/1748-605X/ad38ac
YiQiang Li et al. (2017) mTORC1 signaling is essential for neurofibromatosis type I gene modulated osteogenic differentiation of BMSCs. Journal of Cellular Biochemistry. 10.1002/jcb.26626
Xin-long Ma et al. (2010) Dynamic expression of Runx2, Osterix and AJ18 in the femoral head of steroid-induced osteonecrosis in rats. Orthopaedic Surgery. 10.1111/j.1757-7861.2010.00100.x
