| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Deleted in azoospermia-like; DAZ homolog; DAZ-like autosomal; Deleted in azoospermia-like 1; SPGY-like-autosomal; DAZL; DAZH; DAZL1; DAZLA; SPGYLA |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Sp7/Osterix recombinant protein (Position: A2-E241). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Sp7/Osterix Antibody Picoband® is an antibody for SP7 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SP7 (deleted in azoospermia like); UniProt: Q8TDD2
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 45 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Sp7/Osterix Antibody Picoband® catalog # A02077-1.
Biological background
Biological context: RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts.
Expression and localization notes: cellular localization: Nucleus. Cytoplasm..
Common research applications
- Western blotting (WB): Compare SP7 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SP7 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify SP7-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Transcription factor Sp7, also called Osterix (Osx), is a protein that in humans is encoded by the SP7 gene. This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus. Cytoplasm.
- Research category: Angiogenesis,Cancer,Cardiovascular,Cytokines,Immunology,Innate Immunity,Interleukins,Invasion/Microenvironment
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
