| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sphingosine kinase 1; SK 1; SPK 1; Acetyltransferase SPHK1; SPHK1; SK1; SPHK; SPK |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SPHK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACCB-19 is a rabbit monoclonal antibody that recognizes sphingosine kinase 1 (gene SPHK1) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 42.5 kDa, and the supplier reports an observed band at about 51 kDa.
Sphingosine kinase 1 (SPHK1) phosphorylates sphingosine to produce sphingosine-1-phosphate, a lipid mediator that acts both inside cells and through S1P receptors on the cell surface. By lowering ceramide and raising S1P, SPHK1 generally favors cell growth and survival, and it is studied in inflammation, vascular biology and cancer.
| Target | Sphingosine kinase 1 (gene SPHK1; UniProt Q9NYA1, human) |
|---|---|
| Clone | ACCB-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.5 kDa |
| Observed MW | 51 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human SPHK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sphingosine kinase 1 in the cytoplasm, nucleus, cell membrane and endosome membrane. Tissue expression noted by UniProt (human): Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
