| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein sprouty homolog 2; Spry-2; SPRY2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Sprouty 2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFOH-19 is a rabbit monoclonal antibody that recognizes protein sprouty homolog 2 (gene SPRY2) in human and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 34.7 kDa, and the supplier reports an observed band at about 42 kDa.
Protein sprouty homolog 2 (SPRY2) is a feedback inhibitor of receptor tyrosine kinase signaling that antagonizes FGF-induced ERK1/2 activation. It shapes developmental processes such as lens, limb and lung formation, and it can also protect EGFR from CBL-mediated ubiquitination, so it is studied in growth factor signaling and development.
| Target | Protein sprouty homolog 2 (gene SPRY2; UniProt O43597, human) |
|---|---|
| Clone | AFOH-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.7 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human sprouty 2.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places protein sprouty homolog 2 in the cytoplasm and cell projection.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
