| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Perilipin-2;Adipophilin;Adipose differentiation-related protein;ADRP;PLIN2;ADFP; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Sprouty 4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-SPRY4 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 18S63; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-Sprouty 4 Rabbit Monoclonal Antibody catalog # M04343. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: SPRY4 (Perilipin-2).
- Antibody format: Monoclonal; clone 18S63; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
SPRY4 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): May be involved in development and maintenance of adipose tissue. . Reported cellular localization context: Membrane; Peripheral membrane protein. Tissue expression notes (as provided): Milk lipid globules.
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Heart Disease,Hypoxia,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Metabolism Processes,Pathways and Processes,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate SPRY4 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect SPRY4 expression by Western blot in cell or tissue lysates, Localize SPRY4 by immunofluorescence/immunocytochemistry in cultured cells, Quantify SPRY4-positive cells by flow cytometry in single-cell suspensions, Enrich SPRY4 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 35 kDa; calculated MW: 48075 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 35 kDa
- Cellular localization (provided): Membrane; Peripheral membrane protein.
- Tissue details (provided): Milk lipid globules.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
