| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sequestosome-1;EBI3-associated protein of 60 kDa;EBIAP;p60;Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa;Ubiquitin-binding protein p62;SQSTM1;ORCA, OSIL; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sequestosome-1 (gene SQSTM1) in human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 47.7 kDa, and the supplier reports an observed band at about 62 kDa.
Sequestosome-1 (SQSTM1, also known as p62) is an autophagy receptor that binds polyubiquitinated proteins and LC3 on autophagosomes, delivering protein aggregates for degradation. It also acts as a signaling hub for pathways such as KEAP1-NRF2, and because it is itself degraded by autophagy, p62 is widely used as a marker of autophagic flux.
| Target | Sequestosome-1 (gene SQSTM1; UniProt Q13501, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 47.7 kDa |
| Observed MW | 62 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| IHC (frozen sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human MCF-7 cells, human Jurkat cells, rat liver tissue, rat brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse small intestine tissue, human intestinal cancer tissue, rat small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): human placenta tissue.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with confirmed staining (ICC/IF): human lung cancer tissue.
Immunogen
Synthetic peptide from the N-terminal region of human SQSTM1/p62. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sequestosome-1 in the cytoplasmic vesicle, preautophagosomal structure, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wenchao Wang et al. (2026) mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via Piezo1-NCOA4-ZBP1 axis. Autophagy. 10.1080/15548627.2026.2677183
Jiemei Chen et al. (2022) Cordycepin alleviated metabolic inflammation in Western diet-fed mice by targeting intestinal barrier integrity and intestinal flora. Pharmacological Research. 10.1016/j.phrs.2022.106191
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Anna Stasiewicz et al. (2023) Prevention of UVB Induced Metabolic Changes in Epidermal Cells by Lipid Extract from Microalgae Nannochloropsis oceanica. International Journal of Molecular Sciences. 10.3390/ijms241411302
Ao Xue et al. (2023) Study on the neuroprotective effect of Zhimu-Huangbo extract on mitochondrial dysfunction in HT22 cells induced by D-galactose by promoting mitochondrial autophagy. Journal of Ethnopharmacology. 10.1016/j.jep.2023.117012
Jianchao Si et al. (2023) Tanshinone IIA inhibited intermittent hypoxia induced neuronal injury through promoting autophagy via AMPK-mTOR signaling pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2023.116677
Jing-jing Xing et al. (2019) Ginsenoside Rb3 provides protective effects against cisplatin-induced nephrotoxicity via regulation of AMPK-/mTOR-mediated autophagy and inhibition of apoptosis in vitro and in vivo. Cell Proliferation. 10.1111/cpr.12627
Wenxiu Liu et al. (2025) Aconiti Lateralis Radix Praeparata ameliorates heart failure via PI3K/AKT/Bnip3 pathway. Frontiers in Pharmacology. 10.3389/fphar.2025.1526653
Zuoying Wang et al. (2025) Structural characterization and effect of activating autophagy and regulating oxidative stress of polysaccharide from fibrous roots of Atractylodes chinensis. Bioorganic Chemistry. 10.1016/j.bioorg.2025.108609
