| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sterol regulatory element-binding protein 2; SREBP-2; Class D basic helix-loop-helix protein 2; bHLHd2; Sterol regulatory element-binding transcription factor 2; SREBF2; SREBP2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SREBP2/SREBF2 recombinant protein (Position: R371-L409). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sterol regulatory element-binding protein 2 (gene SREBF2) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 123.7 kDa, and the supplier reports an observed band at about 68 kDa.
Sterol regulatory element-binding protein 2 (SREBP2) is made as an inactive precursor in the endoplasmic reticulum membrane. When cellular sterol levels fall, it is transported to the Golgi and cleaved, releasing a transcription factor that enters the nucleus and switches on genes for cholesterol synthesis and uptake, such as HMGCR and the LDL receptor.
| Target | Sterol regulatory element-binding protein 2 (gene SREBF2; UniProt Q12772, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 123.7 kDa |
| Observed MW | 68 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells, human Jurkat cells, human Raji cells, human HL-60 cells, rat stomach tissue, rat testis tissue, mouse stomach tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): K562 cells, RH35 cells.
Immunogen
Recombinant human SREBP2/SREBF2 fragment (Arg371–Leu409), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sterol regulatory element-binding protein 2 in the Golgi apparatus membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed in adult and fetal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lifang Wang et al. (2024) Cholesterol-lowering effects of rhubarb free anthraquinones and their mechanism of action. European Journal of Pharmacology. 10.1016/j.ejphar.2024.176348
Shuangzhi Chen et al. (2025) Targeting to Intensify M2 Macrophage Polarization Ameliorate Podocyte Lipid Accumulation and Damage by Tea Polyphenols via Activating SIRT1 in the Aged Model Rats With DKD. Food Science & Nutrition. 10.1002/fsn3.70736
