| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2; GalNAc alpha-2,6-sialyltransferase II; ST6GalNAc II; ST6GalNAcII; SThM; Sialyltransferase 7B; SIAT7-B; ST6GALNAC2; SIAT7B; SIATL1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ST6GALNAC2 recombinant protein (Position: Q32-R374). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2 (gene ST6GALNAC2) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 41.9 kDa, and the supplier reports an observed band at about 40 kDa.
ST6GALNAC2 is a sialyltransferase that links sialic acid (alpha-2,6) onto GalNAc residues of O-linked glycans on glycoproteins. Its substrates include the Tn and T antigens, and it is studied in the context of mucin-type O-glycosylation and altered glycan patterns in cancer.
| Target | Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2 (gene ST6GALNAC2; UniProt Q9UJ37, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 41.9 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human ST6GALNAC2 fragment (Gln32–Arg374), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2 in the Golgi apparatus membrane. Tissue expression noted by UniProt (human): Expressed in skeletal muscle, heart, kidney, placenta, lung and leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
