| Field | Specification |
|---|---|
| Target | |
| Alternative names | Steroidogenic acute regulatory protein, mitochondrial; StAR; START domain-containing protein 1; StARD1; STAR; STARD1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human StAR, which shares 85% and 87.5% amino acid (aa) sequence identity with mouse and rat StAR, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects steroidogenic acute regulatory protein, mitochondrial (gene STAR) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 31.9 kDa, and the supplier reports an observed band at about 25 kDa.
Steroidogenic acute regulatory protein (StAR) moves cholesterol from the outer to the inner mitochondrial membrane, where CYP11A1 converts it to pregnenolone. This is the rate-limiting step of steroid hormone synthesis in the adrenal gland and gonads, and StAR mutations cause congenital lipoid adrenal hyperplasia.
| Target | Steroidogenic acute regulatory protein, mitochondrial (gene STAR; UniProt P49675, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 31.9 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human stAR. Sequence identity with the mouse and rat orthologs: 85% and 87.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places steroidogenic acute regulatory protein, mitochondrial in the mitochondrion outer membrane and mitochondrion matrix. Tissue expression noted by UniProt (human): Expressed in gonads, adrenal cortex and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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