| Field | Specification |
|---|---|
| Target | |
| Alternative names | Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human STAT3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AIH-19 is a rabbit monoclonal antibody that recognizes signal transducer and activator of transcription 3 (gene STAT3) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 88.1 kDa, and the supplier reports an observed band at about 88 kDa.
STAT3 is a transcription factor activated mainly by IL-6-family cytokines through gp130 and JAK kinases, and also by growth factors and leptin. Phosphorylated STAT3 dimerizes, enters the nucleus and regulates genes for acute-phase responses, cell survival and proliferation. It shapes Th17 differentiation and is persistently active in many cancers, making it a major research target.
| Target | Signal transducer and activator of transcription 3 (gene STAT3; UniProt P40763, human) |
|---|---|
| Clone | AIH-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 88.1 kDa |
| Observed MW | 88 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human thyroid carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human STAT3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places signal transducer and activator of transcription 3 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jian Zhong et al. (2024) Distinct roles of TREM2 in central nervous system cancers and peripheral cancers. Cancer Cell. 10.1016/j.ccell.2024.05.001
Cuiqing Xie et al. (2026) Synergistic Antitumor Activity and Neuroprotective Effects of FGF1/FGFR Inhibition with Oxaliplatin Chemotherapy. Journal of Advanced Research. 10.1016/j.jare.2026.02.005
Yu Yang et al. (2025) Engineered Panax notoginseng polysaccharide micelles inhibit macrophage polarization and delay the progression of rheumatoid arthritis via JAK2-STAT3 signaling pathway. Journal of Nanobiotechnology. 10.1186/s12951-025-03576-8
Fugen Wu et al. (2024) Enhancing Chemoimmunotherapy for Colorectal Cancer with Paclitaxel and Alantolactone via CD44-Targeted Nanoparticles: A STAT3 Signaling Pathway Modulation Approach. Asian Journal of Pharmaceutical Sciences. 10.1016/j.ajps.2024.100993
Deng Zhicheng et al. (2022) Curcumin and its nano-formulations: Defining triple-negative breast cancer targets through network pharmacology, molecular docking, and experimental verification. Frontiers in Pharmacology. 10.3389/fphar.2022.920514
Ying Shen et al. (2025) Synergistic effects of diammonium glycyrrhizinate and Vitamin D3 against EMT in pulmonary fibrosis through modulating the crosstalk of STAT3/HSP90AA1 and HIF-1α pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2025.120649
Changyu Lei et al. (2025) Pharmacodynamic substances and molecular mechanisms of Tsantan Sumtang alleviating hypoxic pulmonary vascular remodeling. Journal of Ethnopharmacology. 10.1016/j.jep.2025.120585
Yuan Yao et al. (2021) Polyphenol-Rich Extract from Litchi chinensis Seeds Alleviates Hypertension-Induced Renal Damage in Rats. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.0c07046
Shi-Wei Du et al. (2017) Combined Phycocyanin and Hematoporphyrin Monomethyl Ether for Breast Cancer Treatment via Photosensitizers Modified Fe3O4 Nanoparticles Inhibiting the Proliferation and Migration of MCF-7 Cells. Biomacromolecules. 10.1021/acs.biomac.7b01197
Pin Wu et al. (2016) Prognostic role of STAT3 in solid tumors: a systematic review and meta-analysis. Oncotarget. 10.18632/oncotarget.7887
