Anti-STAT6 Rabbit Monoclonal Antibody

SKU:BHA21008042
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Boster Bio
Boster Bio
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Overview
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Anti-STAT6 antibody (Rabbit monoclonal, clone BOC-19, Rabbit IgG). Validated for Western blot, immunofluorescence and flow cytometry. Reactivity: Human. Recognizes signal transducer and activator of transcription 6.
Target STAT6
Clone number BOC-19
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) WB, ICC, IF, Flow Cytometry
Options selector
Catalog no. Size Conjugation
M00523-1 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 uL/vial — Unconjugated
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
      Form: Liquid
      Applications: WB,ICC,IF,Flow Cytometry
      Application details: WB 1:500-1:2000
      ICC/IF 1:50-1:200
      FC 1:50
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target STAT6
Alternative names Signal transducer and activator of transcription 6;IL-4 Stat;STAT6;
UniProt # P42226
Host Rabbit
Clone BOC-19
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunofluorescence
  • Flow Cytometry
Immunogen A synthesized peptide derived from human STAT6
Molecular weight 94.1 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00523-1
Main SKU BHA21008042
Rabbit Monoclonal

Product Overview

Clone BOC-19 is a rabbit monoclonal antibody that recognizes signal transducer and activator of transcription 6 (gene STAT6) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 94.1 kDa, and the supplier reports an observed band at about 94 kDa.

STAT6 is a transcription factor activated by IL-4 and IL-13 receptor signaling through JAK kinases. It drives T-helper 2 differentiation, B-cell class switching to IgE and alternative macrophage activation, so it is central to research on allergy, asthma and type 2 immunity.

Target Signal transducer and activator of transcription 6 (gene STAT6; UniProt P42226, human)
Clone BOC-19
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 94.1 kDa
Observed MW 94 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunocytochemistry / Immunofluorescence 1:50–1:200
Flow cytometry 1:50

Samples with a confirmed band (WB): Daudi cells, HeLa cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Samples with confirmed staining (ICC/IF): HeLa cells.

Immunogen

Synthetic peptide based on the sequence of human STAT6.

Reactivity Notes

The supplier lists reactivity with human. UniProt places signal transducer and activator of transcription 6 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Has this antibody been tested on canine samples?
A.Reactivity with canine samples has not been tested by the supplier. Aligning the immunogen sequence with the canine ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.Is a carrier-free (BSA-free) version available, for example for conjugation?
A.Yes. A carrier-free formulation without BSA, stabilized with trehalose and/or glycerol, can be prepared on request and is compatible with common conjugation chemistries. Please note this when ordering or contact info@biohippo.com. For storage at −20 °C, keep a cryoprotectant such as glycerol in the buffer rather than PBS alone.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 94.1 kDa; observed band at about 94 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human STAT6.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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