| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stathmin;Leukemia-associated phosphoprotein p18;Metablastin;Oncoprotein 18;Op18;Phosphoprotein p19;pp19;Prosolin;Protein Pr22;pp17;STMN1;C1orf215, LAP18, OP18; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Stathmin 1, different from the related mouse sequence by one amino acid, and identical to the related rat sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes stathmin (gene STMN1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 17.3 kDa, and the supplier reports an observed band at about 17 kDa.
Stathmin (STMN1, also called oncoprotein 18) is a small cytosolic phosphoprotein that destabilizes microtubules by sequestering tubulin dimers and promoting microtubule catastrophe. Its activity is switched off by phosphorylation, including at Ser16, linking it to cell cycle progression, neuronal development and cell migration, and it is often highly expressed in cancers.
| Target | Stathmin (gene STMN1; UniProt P16949, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 17.3 kDa |
| Observed MW | 17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human Jurkat cells, human SH-SY5Y cells, human K562 cells, human THP-1 cells, rat brain tissue, rat thymus tissue, mouse brain tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Samples with a confirmed signal (flow cytometry): THP-1 cells, RAW264.7 cells, RH-35 cells.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of human stathmin 1. The peptide sequence is conserved in rat. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places stathmin in the cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Asude Aksoy et al. (2024) The knockdown of stathmin with si-RNA inhibits invasion of mesothelioma. Tissue & Cell. 10.1016/j.tice.2024.102303
Yuqian Wu et al. (2023) Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression. Molecular and Clinical Oncology. 10.3892/mco.2023.2617
