| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Prolactin regulatory element-binding protein;Mammalian guanine nucleotide exchange factor mSec12;PREB;SEC12; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human STIL/SIL recombinant protein (Position: N13-F1287). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-STIL/SIL Antibody Picoband® is an antibody for STIL detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: STIL (Prolactin regulatory element-binding protein); UniProt: Q15468
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 170 kDa, calculated 45468 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-STIL/SIL Antibody Picoband® catalog # A04956-1.
Biological background
Biological context: Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER. .
Expression and localization notes: cellular localization: Endoplasmic reticulum membrane ; Single-pass membrane protein . Nucleus . Concentrates at endoplasmic reticulum exit sites. ., tissue context: Ubiquitous. ..
Common research applications
- Western blotting (WB): Compare STIL levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of STIL in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify STIL-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: SCL-interrupting locus protein is a protein that in humans is encoded by the STIL gene. This gene encodes a cytoplasmic protein implicated in regulation of the mitotic spindle checkpoint, a regulatory pathway that monitors chromosome segregation during cell division to ensure the proper distribution of chromosomes to daughter cells. The protein is phosphorylated in mitosis and in response to activation of the spindle checkpoint, and disappears when cells transition to G1 phase. It interacts with a mitotic regulator, and its expression is required to efficiently activate the spindle checkpoint. It is proposed to regulate Cdc2 kinase activity during spindle checkpoint arrest. Chromosomal deletions that fuse this gene and the adjacent locus commonly occur in T cell leukemias, and are thought to arise through illegitimate V- (D)-J recombination events. Multiple transcript variants encoding different isoforms have been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Endoplasmic reticulum membrane ; Single-pass membrane protein . Nucleus . Concentrates at endoplasmic reticulum exit sites. .
- Tissue details: Ubiquitous. .
- Research category: Epigenetics and Nuclear Signaling,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
