| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Stromal interaction molecule 1, GOK |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-STIM1 (extracellular) Antibody is an antibody targeting Stromal interaction molecule 1, GOK Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IC, IF, IFC, IHC, IP, LCI, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Stromal interaction molecule 1, GOK (also reported as Stromal interaction molecule 1, GOK).
- Immunogen/epitope region: STIM1 expressed on the plasma membrane: Extracellular, N-terminus STIM1 expressed on the ER: Luminal, N-terminus.
- Homology note: Rat, mouse, bovine, canis - identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- KO-validated: yes (validation context may be assay-dependent).
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest.Recently, several key players of the store-operated complex have been identified.3 The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+-binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 molecules are redistributed in punctae underneath the plasma membrane and activate SOCs.Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.5 Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
- Immunoprecipitation (IP): enrich the target for downstream detection or complex analysis (context-dependent).
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Conceptual control: KO/KD samples provide orthogonal support for target assignment when available.
- Provided control suggestions: Negative control: BLP-CC063.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-CC063; Negative control: BLP-CC063.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
