| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stromal interaction molecule 2; STIM2; KIAA1482 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human STIM2 recombinant protein (Position: L458-H726). Human STIM2 shares 89.2% amino acid (aa) sequence identity with mouse STIM2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes stromal interaction molecule 2 (gene STIM2) in human and rat samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 84.0 kDa; the supplier reports observed bands at 98,100 kDa.
STIM2 (stromal interaction molecule 2) is an endoplasmic reticulum Ca2+ sensor related to STIM1 but more responsive to small drops in ER calcium. By activating ORAI channels at the plasma membrane, it helps set basal cytosolic and ER Ca2+ levels and supports store-operated calcium entry, particularly in neurons and immune cells.
| Target | Stromal interaction molecule 2 (gene STIM2; UniProt Q9P246, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 84.0 kDa |
| Observed MW | 98,100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human Jurkat cells, human HepG2 cells, rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Recombinant human STIM2 fragment (Leu458–His726), expressed in E. coli. Sequence identity with the mouse ortholog: 89.2%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places stromal interaction molecule 2 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Expressed in all tissues and tumor cell lines examined. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
