| Field | Specification |
|---|---|
| Target | |
| Alternative names | STE20/SPS1-related proline-alanine-rich protein kinase; Ste-20-related kinase; DCHT; Serine/threonine-protein kinase 39; STK39; PASK; SPAK |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human STK39 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25S16 is a rabbit monoclonal antibody that recognizes STE20/SPS1-related proline-alanine-rich protein kinase (gene STK39) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 59.5 kDa, and the supplier reports an observed band at about 68 kDa.
SPAK (STK39) is a serine/threonine kinase that acts downstream of WNK kinases. Once activated, it phosphorylates cation-chloride cotransporters such as NKCC1, NKCC2 and NCC, controlling their activity. The WNK-SPAK/OSR1 pathway governs cell volume, renal salt handling and blood pressure, and it is studied in hypertension research.
| Target | STE20/SPS1-related proline-alanine-rich protein kinase (gene STK39; UniProt Q9UEW8, human) |
|---|---|
| Clone | 25S16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 59.5 kDa |
| Observed MW | 68 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human STK39.
Reactivity Notes
The supplier lists reactivity with human. UniProt places STE20/SPS1-related proline-alanine-rich protein kinase in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
