| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bile salt sulfotransferase;2.8.2.14;Dehydroepiandrosterone sulfotransferase;DHEA-ST;Hydroxysteroid Sulfotransferase;HST;ST2;ST2A3;Sulfotransferase 2A1;ST2A1;SULT2A1;HST, STD; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SULT2A1, different from the related mouse sequence by seven amino acids, and from the related rat sequence by eight amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sulfotransferase 2A1 (gene SULT2A1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 33.8 kDa, and the supplier reports an observed band at about 30–35 kDa.
SULT2A1 (DHEA sulfotransferase) is a cytosolic enzyme of the liver and adrenal gland that uses PAPS to sulfate DHEA, pregnenolone, bile acids and other hydroxysteroids. Sulfation increases water solubility and excretion and creates circulating steroid reservoirs such as DHEA sulfate, making SULT2A1 important in steroid and bile acid metabolism.
| Target | Sulfotransferase 2A1 (gene SULT2A1; UniProt Q06520, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 33.8 kDa |
| Observed MW | 30–35 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP), human HepG2 cells, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse liver tissue, rat liver tissue, human renal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human SULT2A1. It differs from the mouse sequence at seven residues and from the rat sequence at eight residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sulfotransferase 2A1 in the cytoplasm. Tissue expression noted by UniProt (human): Liver, adrenal and at lower level in the kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
